Contact Heather Vandertol-Vanier If personnel may have been exposed to infectious material due to cabinet failure, then the supervisor must be promptly notified and an incident report completed and the appropriate first aid and medical follow-up action taken.Ĭlean air benches are not Biological safety cabinets and should never be used with pathogens or other materials that are potential aerosol hazards.Place a sign on the cabinet to indicate that it is broken and must not be used.Switch off the power if the motor is making noise.Seal containers, surface decontaminate and remove any biohazardous material.What to do in case of a cabinet failure while working Every time the cabinet is used, record the static pressure on the chart provided by EHS.Do not use an open flame in a biosafety cabinet.If a spill occurs clean it up immediately before continuing work.Place everything at least 4” inside the work area to reduce chance of contamination.Keep activity in the area surrounding the hood to a minimum to reduce the amount of air disruption in the hood.Other helpful hints with biosafety cabinets Refill the bottle of disinfectant, put a new plastic bag in the waste bucket. Spray or swab all interior surfaces with appropriate disinfectant. Use fresh gloves, remove materials to incubator, to biohazard bag, autoclave as appropriate. Remove gloves into contaminated material containerĪllow air purge period with no activity inside (leave blower on!). from clean to work area to discard).ĭO NOT remove hands from work space until procedures are complete and all critical material is secured. Introduce hands into work space, work carefully and methodically (i.e. Record the static pressure on the appropriate paper.ĭon protective clothing, gloves, mask, etc. Place contaminated material container at right rearĪllow air purge period with no activity inside (leave blower on!) Place material such that clean and contaminated items do not meet Introduce only material required to perform procedure (do not block air intake or exhaust grill by over filling the cabinet) Preplan and have all supplies ready for the entire procedure Spray or swab all interior surfaces with appropriate disinfectant (germicide) Biological Safety Cabinets (BSC)īiological safety cabinets should be used when working with pathogens, tissue culture and human samples.Ĭheck air grilles for obstructions, switch on blowerĪllow air to purge workspace for 5 minutes Below are some special considerations for the most commonly used equipment in Level 1 and 2 labs. Using equipment with biohazardous materials requires some special considerations. Standard Operating Procedures for departmental equipment are found on the AFNS safety drive.
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